A, HG induces ICAM-1 expression via IKK, p65, JNK, and c-Jun. HAEC infected with lentiviral particles expressing IKKβ, IKKγ, p65, JNK, or c-Jun shRNA (MOI 0.5 for 48 h) were treated with HG for 12 h and then analyzed for ICAM-1 (A) or VCAM-1 (B) mRNA (12 h) expression by RT-qPCR. Knockdown of respective proteins was confirmed by immunoblotting, and are shown in C. A, B, *P < at least 0.01 vs. NG, †P < at least 0.001 (n=12).