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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Cell Signal. 2012 Oct 17;25(1):359–371. doi: 10.1016/j.cellsig.2012.10.009

Fig. 9. AGE-HSA, oxLDL and AOPPs-HSA induce CIKS expression via Nox4 and ROS.

Fig. 9

A, AGE-HSA (left), oxLDL (middle) and AOPPs-HSA (right) induce CIKS expression. At 70% confluency, the complete medium on HAEC was replaced with EBM-2 (without supplements) for 2h, incubated with 50 μg/ml AGE-HSA, oxLDL, or AOPPs-HSA, and then analyzed for CIKS protein expression by immunoblotting. Results from three independent experiments are summarized in respective lower panels. *P < 0.05 vs. respective unmodified proteins, †P<0.05 vs. respective modified proteins (n=3). B, AGE-HSA, oxLDL and AOPPs-HSA induce CIKS expression via Nox4. HAEC were treated with DPI (10 μM in DMSO for 30 min) or infected with Ad.siNox4 (MOI 100 for 24 h) prior to AGE-HSA ((left; 2 h), oxLDL (middle; 2 h) and AOPPs-HSA (right; 4 h) addition. CIKS expression was analyzed by immunoblotting as in A. Results from three independent experiments are summarized in respective lower panels. *P < 0.05 vs. respective unmodified proteins, †P<0.05 vs. respective modified proteins (n=3).