Table 3.

T cell subclasses, total numbers and ratios comparing samples from patients with necrotising enterocolitis (NEC) with those from non-NEC surgical controls

	Acute NEC (n=18)	Controls (n=30)	p Values*
Total cells	102 882 (56 028; 236 800)†	179 489 (107 768; 262 712)	0.30
Live CD3	3344 (1551; 6086)	2800 (1448; 6525)	1.00
CD3CD4	1438 (571; 4000)	1242 (650; 3049)	0.94
CD4CD45RO	538 (182; 1190)	906 (428; 2274)	0.27
CD3CD8	559 (288; 1448)	333 (172; 932)	0.36
Naive Treg	11 (6; 20)	9 (3; 23)	0.64
Treg‡	44 (18; 80)	100 (33; 164)	0.12
Live CD3 (%)	2.3 (1.1; 5.5)	1.9 (0.6; 4.2)	0.42
CD4/CD3 (%)	46.9 (40.9; 56.6)	48.57 (39.7; 57.3)	0.77
CD45RO/CD4 (%)	46.2 (30.2; 64.6)	65.5 (51.0; 82.3)	0.01
CD8/CD3 (%)	20.5 (11.3; 31.3)	14.5 (10.0; 18.0)	0.04
Naive Treg/CD4 (%)	0.9 (0.6; 1.0)	0.6 (0.2; 1.4)	0.36
Treg/CD4 (%)	2.9 (2.0; 4.6)	6.6 (4.0; 11.8)	0.001
Naive Treg/CD8 (%)	1.5 (0.1; 3.2)	1.2 (0.5; 4.9)	0.62
Treg/CD8 (%)	6.6 (4.5; 9.3)	25.9 (11.0; 47.6)	<0.001

^*Wilcoxon rank sum test.

^†Median (first quartile; third quartile).

^‡Treg defined as viable CD4 CD25high FOXP3high CD127low CD45RO cells.