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. 2013 Mar 15;6(4):598–612.

Figure 5.

Figure 5

Induction of NCI-H661 apoptosis by FER knockdown. A. Relative expression level of FER in lung cancer cell lines, compared with that in NHBE. The error bars indicate the standard deviations. *P < 0.001. B. Western blot analysis after the knockdown of FER by siRNA vectors. Protein from HEK293T transfected with FER expression vectors was used as a positive control. C. Double staining with annexin V-FITC and propidium iodide, followed by flow cytometry analysis to detect apoptotic cells. The percentages in the dot plot results indicate the percentages of apoptotic cells and are shown as a bar graph after triplicate analyses. Cells treated using cisplatin were used as positive controls for apoptosis. The error bars indicate the standard deviations.