Figure 4.

FGFR3-p38 signaling regulates PTX expression. A. p38 inhibitor LY2228820 reduced the PTX-3 secretion induced by LPS in U937 cells.( **, p<0.01; ***, p<0.001), Error bars show data ±standard error. Means were derived from three replicates. B. Western blot analysis of p38 phosphorylation in U937 cells treated by RTK inhibitor Linifanib. U937 cells were treated with FGFR3 inhibitor, Linifanib, with different doses for 24 hrs and centrifuged for western blot analysis. Whole cell lysates were loaded for the expression analysis of phosphorylation of p38 and total p38. C. Optical density analysis of western blot in B. PTX-3 secretion and U937 cell viability was detected by ELISA and MTS assay, respectively. The ELISA/MTS value was calculated as described in Materials and methods.