Immunohistochemical findings. The cholangiocarcinoma (CC) element of the present combined hepatocellular-cholangiocellular carcinoma are positive for four liver stem cell markers; KIT (A), CD56 (B), CD34 (C), and CK14 (D). The CC cell are were positive for antigens showing cholangiocyte lineage; CK AE1/3 (E), CK CAM5.2 (F), CK7 (G), CK8 (H), CK18 (I), CK19 (J), CEA (K), CA19-9 (L), MUC1 (M), MUC2 (N), MUC5AC (O), MUC6 (P), and EpCAM (Q). The cells of CC also show positive some antigens of hepatocellular lineage; HepPar1 (R), AFP (S) CK8 (H), CK18 (I) and CK CAM5.2 (F). The CC cells are positive for EMA (T), CK34BE12 (U), p53 (focal, V) and Ki67-antigen (W) (labeling index= 40%).