Table 2.

A basic outline of major anatomical hippocampal boundaries for 5/12 protocols surveyed by (Boccardi et al., 2011) and used to generate 5 SBHV multi-atlas libraries. Landmarks definitions were adapted from Standard Operating Procedures (www.hippocampal-protocol.net/SOPs) and the original protocol manuscripts.

Protocol (P) Code	Segmentation Method	Hippocampal Boarder						Excluded Anatomy
		Superior	Inferior	Medial	Lateral	Posterior	Anterior
P1	(Haller et al., 1997)	Fx- Fb- or Av-GM interface, IH, AC	WM of PHG and anteriorly: PHG WM/US^*	Tail/Body of the Hc: contour of PHG WM extended by horizontal line towards the AC. Anterior: following incline of PHG WM	Fx, IH, WM of MTL	First slice where Hc appears adjacent to VT	Separation of Hc and Ag (use axial and sagittal views to distinguish)	Fx, Fb, Av, PHG (includes some of the superior-medial PHG)

P2	(Killiany et al.,1993)	Fx- Fb- or Av-GM boundary, IH, AC	WM of PHG and anteriorly: PHG WM/US^*	Posterior/body Hc an oblique line along GM-WM of PHG extending to the AC, and at head of HC following incline of PHG WM	Fx, IH, WM of MTL	Longest length of the Crus of the Fx in coronal view	Slice where the Av differentiates Ag from Hc	Fx, Fb, Av, PHG and portions of medial Sb at the level of the body

P3	(Malykhin et al., 2007)	Fx, Th, AC, Av (most anterior slices)	WM of PHG and anteriorly: PHG WM/US^*	WM of PHG, and when PHG not a horizontal line used an oblique line along PHG WM extending to the AC	Fx, VT, IH, WM of MTL	First slice where an ovoid mass of Hc GM is inferiomedial to VT	Slice along anterior-posterior axis (sagitally) where PHG WM is first visible	Fx and portions of both the Sb and PHG (when PHG WM not a straight line)

P4	^α(Pruessner et al., 2000)	Posterior: Horizontal line that follows the superior PHG WM extending to the AC. Otherwise CSF of QC, IH or Av (most anterior slices)	WM of PHG and anteriorly: PHG WM/US^*	Posterior Hc: Vertical line that follows medial edge of VT, otherwise CSF of AC. Body Hc: 45° line from inferior body to the AC. Head Hc: CSF of AC.	Fx, VT, IH, WM of MTL	First ovoid mass of GM inferiomedial to VT	Slice where either the IH, Ag or Av is present (used axial view to help interpret)	Portions of the medial Sb, Medial GM of PHG at level of body, Fx

P5	^β(Pantel et al.,2000)	Th, AC, Av (most anterior slices)	WM of PHG and anterio WM/US^*	Posterior Hc: QC, Body Hc: Contour of PHG-WM. Anterior Hc: oblique line following PHG WM	Fx, VT, IH, WM of MTL	First ovoid mass of GM inferiomedial to VT	Slice at which head of Hc appears as oval shape below Ag

^α

A pre-processing step in the protocol normalizes brains to Talairach space, which was not performed in the current study.

^β

The updated standardized operating procedure for the hippocampal harmonization procedure includes the alveus and fimbria, which are excluded in the original protocol.

The US commonly widens and becomes a visible landmark as a hypointense band on T1-weighted MRI in atrophic brains.

Key: PHG = parahippocampal gyrus, CSF = cerebrospinal fluid, WM = white matter, Av = alveus, AC = Ambient Cistern, EC = Entorhinal Cortex, IH = Inferior horn of lateral ventricle, Ag=Amygdala, Hc = Hippocampus, MTL = Medial Temporal Lobe, VT = Lateral Ventricular Trigone (also includes atrium of lateral ventricle), Uncal Sulcus = US, Vertical Digitation = VD, Fornix = Fx, Fimbria = Fb, Thalamus = Th, QC= Quadrigeminal Cistern