Fig. 2.

Implementation of the RABV-mediated synaptic tracing in hGFAP-TVA mice. (A) Retroviral and RABV constructs. (B) Adaptation of the method to the hGFAP-TVA mouse line. Temporal profile of TVA expression is indicated by the lower bar. (C) Distribution of the cells targeted by EnvA-pseudotyped RABV in the DG of hGFAP-TVA mice 12 d postinjection (dpi). (Scale bar, 50 μm.) (D) Representative example of RABV-targeted cells in hGFAP-TVA mice comprising of GFAP⁺ horizontal glia (i) and radial glia (ii). Enlargements show single and merged channels of the boxed areas, with arrowheads pointing to the colocalizing immunoreactive signal. (Scale bars, 20 μm.) (E) Example depicting Dcx⁺ newborn neurons targeted by RABV. Enlargement of the boxed area (i) shows the colocalization between GFP and Dcx (arrowheads). (Scale bars, 20 μm.) (F) Quantification of the identity of RABV-targeted cells in hGFAP-TVA mice at 2 and 12 dpi (n = 3 mice). (G) Example of RABV-traced presynaptic neurons at 10 d following injection of G-encoding retrovirus. Enlargements of the boxed area (i) show presynaptic neurons (white arrowheads) surrounding a double-transduced newborn neuron (yellow arrowhead). (Scale bars, 20 μm.)