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. 2013 Mar 4;110(12):4640–4645. doi: 10.1073/pnas.1213971110

Fig. 1.

Fig. 1.

BMP7 promotes transition of CITED1+ progenitors to the SIX2-only compartment. (A) CITED1 (red) localizes to the capsular aspect of the collecting duct (green). (B) Progenitors in the cap lose CITED1 (red) but retain SIX2 (green, arrows). (C) LEF1 (red) is limited to the pretubular aggregate and downstream compartments. (D) A “SIX2 only” progenitor population (green, arrows) resides between the CITED1+ (orange/yellow) and LEF1+ compartments (red). (E) Schematic representation of compartments showing loss of CITED1 as cells differentiate to SIX2-only before entering the LEF1+ pretubular aggregate and renal vesicle compartments. (F) BMP7 treatment results in a loss of CITED1 (red), maintenance of SIX2 (green), and does not promote LEF1 (red, absent). E14.5 Bmp7−/− kidneys are hypomorphic, have a reduced number of CITED1+ progenitors (G and H), and a substantial decrease of the SIX2-only population (I and J, arrows). (K) Quantitation of SIX2-only clusters in E14.5 Bmp7−/− (n = 4, 130 total tips) and wild-type kidneys (n = 6, 510 total tips). Error bars represent average values ± SEM, and the P value is derived from Student t test.