Fig. 2.

Schematic representation of complementation for Pdx1-Hes1 cloned pig embryos with a pancreatogenesis-disabled phenotype using cloned embryos expressing huKO. Primary fibroblast cells as nucleus donor cells for somatic cell cloning were established from a pancreatogenesis-disabled cloned pig with Pdx1-Hes1 transgene expression (A1) and a cloned pig with systemic orange fluorescence conferred by huKO transgene expression (B1). (A2–A4) Host embryos reconstructed by nuclear transfer from male Pdx1-Hes1 transgenic cells yielded pancreatogenesis-disabled piglets. (B2 and B3) Donor embryos were reconstructed by nuclear transfer from female cells expressing huKO. Blastomeres isolated from donor embryos at the morula stage (B4) were inserted into host embryo morulae (A4) to produce chimeric blastocysts (C1) and pigs (C2). (C2) All the chimeric pigs obtained developed into fertile males as a result of intersex chimerism between the male host embryos and female donor embryos. (C3) Sperm of the chimeric boars theoretically originate from male host embryos carrying the Pdx1-Hes1 transgene. After mating of chimeric boars with WT sows (D1), the pancreatogenesis-disabled phenotype of the Pdx1-Hes1 host embryos was transmitted to the next generation (C4).