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List of primers used for backcross breeding and their sequence information and genome specificity.
| Gene (marker) | Primers | Sequence 5′–3′ | Specificity |
|---|---|---|---|
| GSL-ELONG- | PM25 | CCTGTGAGACGTTAATACC | C-genome |
| (PM25 + PM13) | PM13 | GAGCTTGAGTTCTATACGC | |
| GSL-ELONG- | IPM8 | CTTCGAGTAGACATCATGGA | C-genome |
| (IPM8 + IPM7) | IPM7 | AAAGCTATTGTGCGAGGAC | |
| GSL-ELONG+ | Br-MAM1-1F | GTTTCCCTGCGTCATCAGA | A-genome |
| (BraMAM1-1) | Br-MAM1-1R | CTAAGCTCTTCGCATAGCTA | |
| GSL-ELONG+ | AE2F | CAGTCAAATTTACCGCCTT | A-genome |
| (BraAE2) | AE2R | GGTGGCTTTCGCGGACAC |
