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. Author manuscript; available in PMC: 2013 Mar 25.
Published in final edited form as: Biochemistry. 2009 Oct 20;48(41):9858–9870. doi: 10.1021/bi900957e

FIGURE 4.

FIGURE 4

Only enzymatically active forms of the MIF trimer undergo covalent modification by BITC and its active analogues. Six trimeric MIF variants (3 enzymatically active: Wt, R73A, and N110C, and 3 inactive: R73AΔ5, R73AΔ10, and P107) were tested by incubation with 10 μM BITC for 60 min, and the samples were then analyzed by MALDI-TOF/TOF.