FIGURE 4.
Only enzymatically active forms of the MIF trimer undergo covalent modification by BITC and its active analogues. Six trimeric MIF variants (3 enzymatically active: Wt, R73A, and N110C, and 3 inactive: R73AΔ5, R73AΔ10, and P107) were tested by incubation with 10 μM BITC for 60 min, and the samples were then analyzed by MALDI-TOF/TOF.
