Table 2.
Effects of adding methionine to MCDD or MCDHFD on physiological characteristics and plasma markers in C57BL/6J mice (3-week study)
| MCDD | MCDHFD | ||||
|---|---|---|---|---|---|
| Diets Methionine (%) | SD | 0.03 | 0.1 | 0.03 | 0.1 |
| Body weight (g) | |||||
| Initial | 20.9 ± 0.5 | 20.9 ± 0.6 | 21.0 ± 0.5 | 20.8 ± 0.4 | 21.2 ± 0.8 |
| Final | 25.1 ± 0.8## | 16.5 ± 0.6**,## | 22.3 ± 0.7* | 15.1 ± 0.4**,## | 20.1 ± 0.7** |
| Liver/body weight (%) | 5.48 ± 0.11 | 4.21 ± 0.15** | 5.53 ± 0.41 | 5.62 ± 0.14 | 6.66 ± 0.05** |
| Epididymal fat pad/body weight (%) | 1.42 ± 0.16 | 0.98 ± 0.13* | 1.46 ± 0.09 | 0.35 ± 0.04** | 1.25 ± 0.07 |
| ALT (U/l) | 29 ± 5.5 | 333 ± 51** | 124 ± 5.8 | 556 ± 86** | 360 ± 23** |
| AST (U/l) | 65 ± 7.7 | 289 ± 8.4** | 171 ± 16 | 489 ± 82** | 309 ± 9.3** |
| Triglyceride (mg/dl) | 83 ± 13 | 21 ± 3.1** | 29 ± 3.2* | 45 ± 21 | 56 ± 4.3 |
| Total cholesterol (mg/dl) | 82 ± 5.3 | 31 ± 7.6** | 54 ± 3.2** | 46 ± 9.0** | 48 ± 3.7** |
ALT, alanine aminotransferase; AST, aspartate aminotransferase; SD, standard diet; MCDD, methionine-/choline-deficient diet; MCDHFD, methionine-/choline-deficient high-fat diet.
Values are expressed as means ± SEM for 3 mice in each group.
*
P < 0.05,
**
P < 0.01, compared with the SD (control).
##
P < 0.01, compared with the initial body weight.