Figure 1. VIP is present in nNOS+ nerves of WT mice but not VIP−/− mice.
A, Dual labeling of nerves with anti-nNOS (red, Aa) and anti-VIP (green, Ab) antibodies reveals co-localization of VIP and nNOS in WT mice (Ac). Note more punctate nature of VIP labeling. B, nNOS labeling is present in the VIP−/− mouse (Ba,Bc) while VIP labeling is absent (Bb,Bc).