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. 2013 Jan 21;591(Pt 6):1489–1506. doi: 10.1113/jphysiol.2012.247684

Figure 5. The VIP receptor antagonist VIP6–28 abolishes NNNP relaxation in WT mice to reveal non-cholinergic contraction.

Figure 5

Aa, sample traces showing NNNP relaxations elicited with 20 Hz EFS for 4–30 s in WT mice. Ab, sample traces from the same muscle following addition of 30 μm VIP6–28. The NNNP relaxation is blocked, revealing non-cholinergic contraction. B, summary graph of the contractile activity during (Ba) and after 20 Hz EFS (4–30 s; Bb) in WT muscles. The relaxation occurring in the presence of MRS2500 and l-NNA (n = 9) was blocked by VIP6–28 (n = 5) and replaced with contraction (Ba; *p<0.05). Post-stimulus relaxation (n = 11) was also blocked by VIP6–28 (n = 5; Bb). One-way ANOVA with Tukey's post hoc multiple comparison. Shown are mean values ± SEM. Both 100 μm l-NNA and 1 μm MRS2500 were present throughout.