Figure 2. Fibre typing of segments of individual human skeletal muscle fibres.

In A, lanes 2, 3 and 8 contain type I fibres; lanes 1, 4, 6, 7 and 9 contain type II fibres; and lane 5 is an example of an empty sample. B, fibre type-specific differences in sarco(endo)plasmic reticulum Ca²⁺-ATPase isoform 1 (SERCA1) protein expression (type I, n = 49 and type II, n = 52) were determined by Western blotting on segments of human single muscle fibres. Student's unpaired t test was performed to detect the differences. ***Significant difference (P < 0.001) between protein expression in type I and type II fibres. Data are expressed as means + SEM.