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. 2013 Jan 7;591(Pt 6):1551–1561. doi: 10.1113/jphysiol.2012.245944

Figure 1. Mitochondrial isolation cleanliness checks.

Figure 1

A, to ensure that our mitochondrial isolation procedures yielded sample devoid of nuclear contamination we loaded 5, 10, 20 30 μg of isolated mitochondrial protein (subsarcolemmal (SS) and intermyofibrillar (IMF)) and probed for H2B (nuclear protein) and COXIV (mitochondrial protein). This check was performed on the same membrane and indicates that our isolation procedure yields highly purified mitochondria. B, to confirm that PGC-1α exists within both the SS and the IMF fraction, 10 μg of mitochondrial protein was loaded and detected in concert with other mitochondrial proteins and the absence of non-mitochondrial proteins. Thirty micrograms of muscle homogenate protein was loaded.