Figure 2. In human skeletal muscle, exercise promotes PGC-1α translocation to subsarcolemmal mitochondria.

A, representative control blots to ensure purified mitochondrial samples. B, following an acute bout of exercise at 60% for 120 min, PGC-1α is increased in subsarcolemmal (SS) mitochondria 3 h post-exercise. C, in contrast, Tfam is not increased in either mitochondrial population; n = 8 for all independent experiments. Data are expressed as mean ± SEM. *P < 0.05, significantly different from rest. Ten micrograms of mitochondrial protein was loaded for all conditions. IMF, intermyofibrillar mitochondria.