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. 2013 Mar 19;9(6):281–285. doi: 10.6026/97320630009281

Figure 1.

Figure 1

Cloning of α-amylase gene from S.aureus ATCC 12600. A. PCR amplification of α-amylase gene using α-amylase primers from the S. aureus ATCC12600 following the method described. Lane M molecular size marker obtained from Bangalore genei pvt ltd, lane L1 and L2 PCR amplified products. B. Schematic representation of pQE 30 plasmid vector, C. Electrophoretogram showing the expression of recombinant α-amylase from PLAM1clone in SDS-PAGE (10%). Lane M: Molecular weight markers obtained from Bangalore Genei Pvt ltd. Lane 1: induced cell lysate of PLAM1 clone Lanes 2 : uninduced cell lysate of PLAM1 clone. Lane 3: pure recombinant α-amylase eluted from nickel metal chelate chromatographic column.