Table II.
The pBR322 DNA damage induced at different concentrations of peroxynitrite or by benzene metabolites in the presence of the NO-releasing compound (DEA-NO).a
| Compounds | Percent of single-strand DNA breaks at different concentrations of peroxynitrite or benzene metabolites (mean ± SD) | AUCb 0.0–1.0 mM (mean ± SD) |
||
|---|---|---|---|---|
| Concentration of peroxynitrite or benzene metabolites | 0.1 mM | 0.5 mM | 1.0 mM | |
| Peroxynitrite | 42.3±18.9 | 81.2±4.5 | 82.4±2.7 | 72.4±5.9 |
| HQ+•NO | 54.0±12.2 | 77.7±7.6 | 78.0±6.3 | 70.2±6.2 |
| BQ+•NO | 22.8±3.3c | 47.5±4.9d | 53.2±5.8d | 40.6±4.3d |
| CAT+•NO | 24.3±6.9c | 39.6±6.7d | 38.1±6.0d | 33.7±6.3d |
| BT+•NO | 23.3±5.5c | 57.4±2.4d | 80.7±8.9 | 52.0±2.7d |
| Phenol+•NO | 4.3±3.3d | 3.3±2.3d | 3.6±1.3d | 3.6±2.3d |
| Benzene+•NO | 0.1±0.1d | 2.8±1.6d | 0.1±0.1d | 0.7±0.4d |
| HQ | 1.1±0.8d | 1.9±0.7d | 2.8±2.0d | 1.9±0.7d |
| BQ | 0.1±0.1d | 0.7±1.2d | 1.1±1.0d | 0.6±0.7d |
| CAT | 2.4±2.2d | 1.7±1.6d | 2.3±1.3d | 2.0±1.6d |
| BT | 20.2±5.3d | 42.6±5.5d | 53.3±3.6d | 37.7±4.5d |
| Phenol | 1.1±1.7d | 1.6±1.5d | 1.4 ±1.4d | 1.4±1.3d |
| Benzene | 0.3±0.4d | 1.3±0.5d | 1.3±0.8d | 1.0±0.9d |
a
Incubations included pBR322 DNA (100 ng), DEA-NO (0.5 mM), ring-hydroxylated benzene metabolites or peroxynitrite (0.01–1mM).
b
AUC, area under curve. The values shown in the column AUC include concentrations of 0.01, 0.1, 0.2, 0.5 and 1.0 mM that are shown in Fig. 4.
c
Indicates a significant difference from peroxynitrite alone at p=0.05.
d
Indicates a significant difference from peroxynitrite alone at p=0.01.