Figure 2.

Trichostatin A Does Not Decrease the Expression of Hsp90 or Key Drug-Resistance Determinants

(A) Treatment of C. albicans or S. cerevisiae with trichostatin A (TSA) does not reduce Hsp90 gene expression. Strains were grown in rich medium to log phase without (−) or with TSA. Transcript levels of gene(s) encoding Hsp90 were measured by quantitative RT-PCR and normalized to GPD1 (C. albicans) or ACT1 (S. cerevisiae). Levels are expressed relative to the untreated sample, which is set to 1. Data are mean ± SD for triplicates.

(B) Treatment of S. cerevisiae or C. albicans with TSA does not alter Hsp90 protein levels. Cultures were grown as in (A). Total protein was resolved by SDS-PAGE, and blots were hybridized with α-Hsp90 or α-tubulin.

(C) TSA does not block the expression of azole-resistance determinants. Transcript levels of C. albicans or S. cerevisiae erg3 mutants were measured by quantitative RT-PCR after growth to log phase with no treatment, TSA, fluconazole (FL), or the combination of TSA and FL. Transcript levels are normalized as in (A).

See also Table S2.