Figure 5.

Inhibition of KDACs Impairs Calcineurin Activation and Blocks the Interaction between Hsp90 and Calcineurin

(A) Geldanamycin (GdA) and trichostatin A (TSA) block calcium-induced calcineurin activation. Log phase cultures of a S. cerevisiae strain containing a 4×CDRE-lacZ reporter or a C. albicans strain harboring a UTR2p-lacZ construct were untreated (−), treated with 200 mM CaCl2 (Ca), treated with Ca and GdA, or treated with Ca and TSA. Data are mean ± SD from technical triplicates and are representative of biological duplicates. ^∗p < 0.001.

(B) Deletion of HDA1 and RPD3 blocks the physical interaction between Hsp90 and calcineurin. Protein from S. cerevisiae strains harboring a HA-tagged catalytic subunit of calcineurin (Cna1) was immunoprecipitated with anti-HA agarose beads, resolved by SDS-PAGE, and blots were hybridized with α-HA or α-Hsp90 antibodies. Experiment was quantified using ImageJ, and data are mean ± SD of biological duplicates.