Figure S2.

K27 and K270 Point Mutations in Hsp90-Mimicking Acetylation or Deacetylation Do Not Alter Fluconazole Susceptibility, Related to Figure 4

To test the functional importance of candidate acetylated lysines, site-directed mutagenesis was performed to mutate K27 and K270 of Hsp90 to mimic permanent acetylation (lysine (K) to glutamine (Q)) or deacetylation (lysine (K) to arginine (R)). These mutant alleles were expressed as the only source of Hsp90 in the cell. We tested the impact of these mutations on fluconazole (FL) susceptibility in an MIC assay. Cultures were grown in YPD at 30°C for 48 hr and growth was measured as in Figure 1.