Figure 1.

Half-Embryo Development

(A) Developmental progression of half embryos (upper row, equivalent stages in parentheses) and whole embryos (lower row).

(B) Time-lapse imaging of half-embryo development. Lineage diagram: A, apoptosis; I, inside cell; O, outside cell; 1, wave 1 internalization; 2, wave 2 internalization.

(C) Compaction, division, and cavitation occur with the same timing in half and whole embryos imaged side by side (merged differential interference contrast and GFP frames from Movie S1).

(D) Proportions of first- and second-wave internalizations in whole embryos (from Morris et al., 2010) and half embryos.

(E) Time-lapse imaging of cell internalization by asymmetric division (whole embryo) and engulfment (half embryos). Time: hours:minutes.

(F) aPKCζ immunostaining of half and whole embryos in the 4(8)- to 8(16)-cell-stage transition. Scale bars: 50 μM (B) and 25 μM (D and E).

(G) Time-lapse imaging of cell internalization by asymmetric division (whole embryo) and engulfment (half embryos) in embryos expressing E-cadherin-GFP.

(H) Final fates of cells derived from the first and second waves in half embryos.

All error bars indicate standard error. See also Figure S1 and Movies S2, S3, and S4.