Figure 5.

Satb1 Deletion Results in Impaired Differentiation of SST⁺ Cortical Interneurons

(A–F) In situ hybridization histochemistry of P15 cortical sections from wild-type (A, C, and E) and Satb1-deficient (B, D, and F) mice with riboprobes specific for Gad1 (A and B), Lhx6 (C and D), and Sst (E and F).

(G) Quantification of Gad1-, Lhx6-, and Sst-expressing cells in the somatosensory cortex (S1BF, bregma 0.38 mm) of wild-type (white bars) and Satb1-deficient mice (black bars).

(H–K) Immunofluorescence of P15 cortical sections from wild-type (H and J) and Satb1-deficient (I and K) mice with antibodies specific for SST (H and I) and PV (J and K).

(L) Quantification of SST⁺ and PV⁺ cells in the somatosensory cortex (S1BF, bregma 0.38 mm) of wild-type (white bars) and Satb1-deficient mice (black bars).

(M–P) immunofluorescence of P15 cortical sections from wild-type (M and O) and Satb1-deficient (N and P) mice with antibodies specific for CR (M and N) or NPY (O and P).

(Q) Percentages of CR⁺ and NPY⁺ cells in wild-type (white bars) and Satb1-deficient cortex (black bars).

(R and S) In situ hybridization histochemistry of P15 cortical sections from wild-type (R) and Satb1 mutant (S) mice with a Crh-specific riboprobe.

(T) Quantification of CRH⁺ neurons in the layers of the somatosensory cortex (S1BF, bregma 0.38 mm) of wild-type (white bars) and Satb1-deficient mice (black bars).

n = 3 animals per each interneuron marker analysis, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 for each test (Student’s homoscedastic t test with two-tailed distibution). See also Figures S4, S5, and S7.