Figure S1.

Manipulation of Satb1 Expression in the MGE, Related to Figures 3, 4, and 6

(A) Schematic representation of MGE electroporation and culture of forebrain slices from E14.5 mouse embryos.

(B–C2) Sections of slices in which the MGE was transfected with pRFP (B–B2) or pSATB1-RFP (C–C2) and immunostained 24 hr later for RFP (B1, C1) or SATB1 (B2, C2). (B) and (C) are overlays of (B1) and (B2) and (C1) and (C2), respectively. SATB1 is detected only in sections from slices electroporated with pSATB1-RFP vector.

(D) Western blot analysis of protein extracts from P19 embryonal carcinoma cells cotransfected with pSATB1-RFP and constructs expressing SATB1- or scrambled (control) shRNAs. Note the efficient downregulation of SATB1 upon cotransfection of Satb1-specific shRNAs.