Figure S3.

Expression of Satb1 in the MGE Inhibits the Tangential Migration of Interneurons, Additional Results Related to Figure 3

(A) Schematic presentation of forebrain slices from E14.5 mouse embryos electroporated in the MGE and cultured for 2 days.

(B) Image of a slice which was electroporated with the control vector pRFP (transfected cells shown in red), cultured for two days and counterstained with DAPI (blue). B1 shows an enlargement of the box B1 in (B). Inset in (B1) shows images of tangentially migrating interneurons with a prominent leading process.

(C) Image of a slice electroporated with the pSatb1-RFP vector and cultured in parallel to the control slice shown in (B). Note the absence of tangentially migrating SATB1⁺ interneurons. C1, shows an enlargement of the box C1 in (C).

(D and E) Schematic of a cultured slice 48 hr after electroporation. The effect of SATB1 overexpression on tangential migration of interneurons was quantified by counting RFP⁺ cells within the indicated boxed area in the pallium. Data obtained are shown as a histogram in (E); n = 12 slices per condition, ^∗∗∗p < 0.001 (Student’s homoscedastic t test with two-tailed distribution).