Figure S4.

Analysis of Interneuron Markers in P15 Wild-Type and Satb1-Deficient Mice, Additional Results Related to Figure 5

(A) Quantification of GAD1⁺, LHX6⁺ and SST⁺ interneurons in the motor (M1, bregma 0.38 mm), somatosensory (S1, bregma −1.94 mm) and visual (V1, bregma −2.92 mm) cortex of wild-type (white bars) and Satb1-deficient (black bars) mice. The number of Sst mRNA-expressing interneurons is significantly reduced in all areas examined.

(B–E) Immunofluoresence on P15 cortical sections from wild-type (Satb1^+/+; B and D) and Satb1-deficient (Satb1^−/−; C and E) mice with antibodies specific for VIP (B and C) and nNOS and SST (D and E). n = 3 animals per genotype, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 (Student’s homoscedastic t test with two-tailed distribution).