Figure 4.

Dissociated cells or cell clusters overlying disruptions in MM near lymphoid follicle. A normal colon tissue section from a node-positive case was double immunostained for CK AE1/3 (red) and collagen IV (brown). The circle identifies the low magnification view of the structure in B, and the circle in B identifies the gap in the MM. Arrows identify solid epithelial cell clusters without a distinct BM overlying the focal disruption in the MM. Arrowheads identify the MM. Stars identify normal epithelial structures with a distinct lume (stars). Note that a vast majority of the epithelial structures distant from lymphoid follicles are uniform in size with a distinct lumen, while isolated solid cell clusters without a lumen and the surrounding BM are exclusively seen overlying the focal disruption in the MM. A: 80X. B: 400X