Figure 5.

Tcf15 Drives the Transition to Primed Epiblast and Suppresses Primitive Endoderm Differentiation

(A) qPCR analysis of dox-inducible Tcf15-E47 ESCs after 4 days in N2B27 + LIF + BMP4.

(B) Dox-inducible Tcf15-E47 ESCs after 4 days in N2B27 + LIF + BMP4. Second panel shows cells that received a 24 hr pulse of dox during the first day.

(C) Dox-inducible Tcf15-E47 ESCs after 4 days in N2B27. Treatment with dox eliminates spontaneous differentiation into primitive-endoderm-like cells.

(D) qPCR analysis of dox-inducible Tcf15-E47 cells during differentiation in N2B27.

(E) qPCR analysis of dox-inducible Tcf15-E47 cells after transfer to EpiSC conditions.

All data are represented as mean ±SD. See also Figures S3 and S4.