Figure 7.

Tcf15 Downregulates Nanog and Upregulates Otx2

(A) Western blot analysis of flag-Tcf15-E47 or parental cells in ESCs at various time points after addition of dox.

(B) Genes that respond most strongly to Tcf15-E47 after 12 hr dox treatment. Note that Tcf3 refers to the E2A gene and detects the E47 transgene in this experiment.

(C) Fold change in all genes within the microarray probe set. Nanog is highlighted in red and Otx2 is highlighted in green.

(D) qPCR validation showing downregulation of Nanog pre-mRNA and Otx2 mRNA in response to dox-mediated induction of Tcf15-E47 in ESCs.

(E) qPCR analysis showing the kinetics of Tcf15, Nanog, and Otx2 expression during the transition toward EpiSC.

(F) Model. We propose that Tcf15 suppresses Nanog and activates Otx2 to drive pluripotent cells away from naive pluripotency and toward epiblast.

All data are represented as mean ±SD.