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. Author manuscript; available in PMC: 2013 Mar 25.
Published in final edited form as: Immunity. 2009 Jan 16;30(1):92–107. doi: 10.1016/j.immuni.2008.11.005

Figure 7. Effects of IL-23 and IL-12 Blockade on Th17 Precursors in Transfer Model of Colitis.

Figure 7

(A) Th17-polarized T cells were generated from naive Il17fThy1.1/Thy1.1 CD4+ T cells with Il12b−/− APCs and anti-CD3 (5 μg/ml) as in Figure 1, in the absence (Th17 -IL-23) or presence (Th17 +IL-23) of 5 ng/ml IL-23. Thy1.1+ (IL-17F+) cells were isolated by FACS sorting and 3 × 105 cells were transferred into congenic Rag1−/− mice as indicated. Recipients were administered 100 μg of anti-IL-23R, anti-IL-12p40, or isotype control via i.p. injection in saline 1 day prior and 1 day after T cell transfers and once weekly thereafter (five total doses). Mice were sacrificed 4 weeks after transfer and analyzed histologically and scored for development of colitis.

(B) Mice that received Th17 -IL-23 (IL-17F+) T cells and treated as in (A) were sacrificed 4 weeks after transfer, and cells recovered from MLNs were stimulated ex vivo with PMA ionomycin and analyzed for CD4 and intracellular IL-17A and IFN-γ by FACS. Data are the means from five mice in each treatment group. *p < 0.05 and **p < 0.01 versus control treatment group. Data are representative of two similar experiments.