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. Author manuscript; available in PMC: 2013 Mar 25.
Published in final edited form as: J Am Chem Soc. 2011 Mar 15;133(13):4865–4873. doi: 10.1021/ja1093919

Figure 3.

Figure 3

Time courses of 630 nm absorption changes due to reactions of a) 14 μM CuI WT azurin mixed with 405 μM CuII C112D azurin and b) 13.5 μM CuI azurin mixed with 270 μM CuII C112D/M121L azurin. Both traces represent reactions at 25 °C in 100 mM sodium phosphate, pH 7.0. Fits to reversible bimolecular reactions are overlaid in black. Fitting parameters to 5 half-lives for a): 405 μM CuII C112D azurin mixed with 9.6 μM CuI WT azurin (14 μM by A280) at 298 K. k12 = 360 M−1s−1 with K12(298 K) = 0.0081. b): 270 μM CuII C112D/M121L azurin mixed with 13.5 μM CuI WT azurin (13.5 μM by A280) at 298 K. k12 = 36,200 M−1s−1 with K12(298K) = 0.41.