Figure 1. Inhibition of caspase-9-mediated nuclear damage by Bcl-2^D/A or Bcl-xL^D/A.

(A) Control H9-iCasp9 cells, or H9-iCasp9 cells expressing wild type Bcl-2 or Bcl-xL, or mutant Bcl-2 or Bcl-xL (Bcl-2^D/A or Bcl-xL^D/A), were cultured 6 h in the presence or absence of 10 nM of the divalent FK506 analog, AP20187, as a chemical-inducer of dimerization (CID) to activate iCasp9 [6,24]. Nuclear morphology was visualized by staining with DAPI and mitochondria were visualized using MitoTracker Deep Red. (B) Cells as in (A) were cultured for 12 h in the presence or absence of 10 nM of CID. Sub-diploid DNA content was measured by staining with propidium iodide. Results are representative of at least two experiments.