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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Biochemistry. 2013 Feb 15;52(9):1568–1582. doi: 10.1021/bi301570m

Figure 5.

Figure 5

Representative DLS and SAXS data. DLS size distribution histograms are shown for Ms sGC NT19 (A) and Ms sGC NT21 (B) (2 mg/ml each). The histograms were derived from a regularization analysis of the autocorrelation curve (insets). The intensity autocorrelation (IA) was evaluated as a function of time delay between 1 μs and 0.1 s. Ms sGC NT19 displays 8.0% polydispersity, while NT21 displays 9.6% polydispersity. C) Guinier plots for all NT19 and NT21 complexes, computed from merged data sets (1.3 mg/ml and 5.0 mg/ml, respectively, for the low angle and high angle data). D) Overlay of the NT19 and NT21 pair distance distribution functions p(r), computed using GNOM. E) Overlay of scattering curves for NT19, NT19 + NO, and NT19 + CO and BAY 41-2272. The curves show very little difference in scattering intensity distribution, indicating little change in shape upon complex formation. F) A similar overlay of for NT21 complexes (uncorrected), which also indicate little change in scattering intensity upon complex formation.