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Mutant Twinkle induces autophagy in the SNC. (A) Immunohistochemistry of LC3 in the SNC. (B) LC3 mean density was increased in the SNC (WT = 4, TG = 4, P = 0.000977). (C and D) LC3-II was found in PC12 cells transfected with Twinkle duplicate after 48 h, not in cells transfected with Twinkle WT. *P≤ 0.05.
