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. 2013 Mar 25;8(3):e59896. doi: 10.1371/journal.pone.0059896

Figure 3. SP-A fails to inhibit the LPS-induced co-loclization of TLR4/EEA1 in β-arrestin 2−/− AM.

Figure 3

A + B and D + E, Representative IF for TLR4 localization in primary AM from WT (A and D) and β-arrestin 2−/− mice (B and E) treated with LPS (100 ng/ml), SP-A (40 µg/ml), or both as indicated. Images are representative of at least three independent experiments in which over 80% of the cells had similar staining patterns. Early endosomes were stained with EEA1 and the post-Golgi compartment was stained with Vti1b. Arrows indicate the area of co-localization. Scale bars, 10 µm. C and F, Analysis of TLR4 and EEA (C) or TLR4 and Vti1b (F) co-localization. Values are expressed in percentage ± SEM of at least three independent experiments with at least 50 cells per condition. Data were statistically analyzed by one-way ANOVA with Bonferroni's posttest. *p<0.05; ***p<0.001.