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. 2013 Mar 25;8(3):e59792. doi: 10.1371/journal.pone.0059792

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© 2013 Barrow et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: HT29 glycan expression in cell response to C1GalT siRNA or control siRNA. After treatment of the cells with C1GalT siRNA or control non-targeting siRNA (con-siRNA), cellular expressions of TF, Tn, sialyl-Tn and GSL-II binding (GlcNAc-, Core 3-associated glycans) were assessed by slot blots with monoclonal antibodies against TF (TF5), Tn (HB-Tn1), sialy-Tn (HB-STn1) or with biotin-GSL-II. Parallel blots were probed with antibody against β-actin for equal protein loading. Duplicate assessments are shown for each blot. B: Quantification of the expressions of cellular TF, Tn, sialy-Tn and GSL-II binding (GlcNAc-, Core 3-associated glycans) in HT29 cell response to C1GalT siRNA. Densities of the slots blots were quantified* and the glycan expressions are expressed as percentage change to the non-siRNA control after normalization with protein loading. *The blot densities of TF expression from untreated and C1GalT siRNA treated HT29 cells were 5004 and 715; Tn 1314 and 4345; sialyl-Tn 1634 and 4868; GSL-II binding (Core 3) 489 and 1156 and tublin 1898 and 1928.