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. 2013 Mar 25;8(3):e59792. doi: 10.1371/journal.pone.0059792

Figure 3. Effect of C1GalT siRNA on cellular TF, Tn, sialyl-Tn and Core 3 glycan expressions in SW620 cells.

Figure 3

Sub-confluent SW620 cells cultured in 8-well glass chamber slides were treated without or with C1GalT siRNA or control non-targeting siRNA for 48 hr before the expressions of cell TF, Tn, sialyl-Tn and GSL-II binding (Core 3-associated) glycans were assessed by fluorescence immunohistochemistry using biotin-PNA, biotin-GSL-II or antibodies against Tn (HB-Tn1) or sialyl-Tn (HB-STn1). Representative images are shown.