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. 2013 Mar 25;8(3):e60507. doi: 10.1371/journal.pone.0060507

Figure 4. Comparison of protective antibody responses.

Figure 4

Balb/c mice were vaccinated i.m. with electroporation three times at two week intervals with 20 µg each of DNA expressing L1 or L1+L2 of HPV6, HPV16, and HPV18, either individually, or together at the same site or each at a different site, or s.c. with Gardasil. Serum samples were collected two weeks (A,B) and three months (C) after the last vaccination to test their protective efficacy in vivo and in vitro. Naïve Balb/c mice (5 per group) were passively immunized i.v. with 20 µl of pooled serum. Mice were challenged intra-vaginally with HPV16 (A,C) or HPV6 (B) PsV carrying a luciferase reporter construct. Three days later, luciferin was administered intra vaginally, and bioluminescence imaging was performed.