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. 2013 Mar 25;8(3):e60507. doi: 10.1371/journal.pone.0060507

Figure 5. Interaction between HPV18 L1 with L1 of both HPV6 and HPV16.

Figure 5

To test for interaction between L1 proteins of different HPV genotypes, 293TT cells were transfected with empty DNA vector (V), or vector expressing HPV6 L1, HPV16 L1, or HPV18 L1 individually (6L1, 16L1, 18L1, respectively), or all three together (Mix). Two days later, the cells were harvested and lysed. HPV18 L1 was immune-precipitated from lysates using the conformationally-dependent and neutralizing monoclonal antibody H18.F8. The presence of HPV6 L1, HPV16 L1 and HPV18 L1 in the immune-precipitates was detected by Western blotting with H6.C6 (left panel), H16.O7 (middle panel), and H18.E20 (right panel) respectively.