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. 2013 Mar 25;8(3):e59441. doi: 10.1371/journal.pone.0059441

Figure 1. IL-33 stimulated macrophages to produce IL-13 in vitro.

Figure 1

A, B, Time- and concentration-dependent upregulation of IL-13 mRNA expression (A, qPCR) or IL-13 secretion from cell culture supernatants (B, ELISA) in BMDM stimulated with IL-33. C, After IL-33 stimulation, BMDM were stained with antibodies, and analyzed by FACS for surface F4/80 and intracellular IL-13 expression. D, BMDM from WT, STAT6−/−, IL-4Rα−/−, or IL-4Rα−/−Rag2−/− mice were stimulated with IL-33, and analyzed for IL-13 mRNA expression (72 hours) by qPCR. Data shown in bar graphs are the mean ± s.e.m and representative of at least two independent experiments.