Established model of glioblastoma development in the offspring of rats exposed prenatally to the mutagen N-ethyl-N-nitrosourea (ENU)
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Solid tumor formation in vivo (brain tumor model induced by carcinogen)
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AMPK is strongly activated during early stage of tumorigenesis in vivo.
|
22
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LKB1-null MEFs transformed by oncogene
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Anchorage-independent growth (soft agar)
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LKB1-null MEFs are resistant to oncogene-induced transformation.
|
23
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AMPKα1α2-double knockout MEFs transformed by H-Rasv12
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Solid tumor formation in vivo (xenograft)
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AMPKα1α2-double knockout MEFs are severely impaired in their ability to form tumors in vivo.
|
24
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AMPKα1α2 double knockout MEFs transformed by H-Rasv12
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Matrix detachment (cell viability)
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AMPK confers anoikis resistance in transformed cells
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25
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Pancreatic cancer cell lines
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Glucose deprivation (cell viability), anchorage-independent growth (soft agar) and solid tumor formation (xenograft)
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AMPK knockdown using siRNA sensitizes cell death during glucose deprivation and also impairs anchorage-independent growth and solid tumor formation.
|
26
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Glioblastoma cell lines
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Glucose deprivation (cell viability and spheroid migration)
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AMPK signaling promotes cell survival and migration during glucose deprivation
|
27
|
Prostate cancer cell lines
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Non-stress conditions (migration?)
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CaMKK2 is increased by androgen and mediates androgen dependent regulation of cell migration through AMPK.
|
28
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Prostate cancer cell lines
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Non-stress conditions (cell proliferation and apoptosis)
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Inhibition of AMPK by RNAi or compound C decreases cell proliferation and induces apoptosis.
|
29
|
Prostate cancer cell lines
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Lipid deprivation (caspase activity and cell mass) and solid tumor formation (xenograft)
|
siRNA screening in prostate cancer cell lines under lipid deprivation identified AMPKβ1 subunit as an essential gene for survival.
|
30
|
Prostate cancer cell lines
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Glucose deprivation (cell viability)
|
AMPK promotes prostate cancer cell survival during glucose deprivation.
|
31
|
Hepatocarcinoma cells isolated from mouse primary tumor induced by myc, Akt,p53−/−
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Solid tumor formation (orthotopic transplantation into liver capsule)
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Myc-driven tumorigenesis requires AMPK activity that promotes mitochondrial metabolism.
|
32
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Pancreatic cancer cell line and glioblastoma cell line |
Matrix detachment (cell viability) and anchorage-independent growth (soft agar) |
KSR2 promotes metabolic activity, anoikis resistance and anchorage-independent growth via AMPK. |
33 |