Fig. 1.

Schematic representation of the two experiments. In experiment 1, all the oocytes were vitrified with V1; half were warmed with W1 and the other half with W2. For some V1W2 oocytes, development was compared with fresh sibling oocytes. In experiment 2, half of the oocytes were vitrified with V1 and the other half with V2. All the oocytes were warmed with W2. After warming Polscope analysis was performed. Preimplantation development was followed in both experiments until day 6 followed by immunocytochemical analysis