Figure 2.

GnRH Stimulation of pCREB Levels Is Attenuated by a DNPKA Inhibitor. LβT2 cells in static culture were transiently transfected with 2 μg total DNA: 2 μg empty vector (EV, black solid bars); 1 μg empty vector in combination with 1 μg DNPKA (EV:DN, gray shaded bars); or 2 μg DNPKA alone (DN, white solid bars). Cells were treated 40 hours after transfection with 10 nM GnRH for 0, 10, or 30 minutes before harvest. Bar graphs show relative pCREB levels (mean ± SEM from three independent experiments, each performed in triplicate, normalized to CREB). Western immunoblot shown below bar graph is from one representative experiment. Significant differences (P < .05), measured by one-way ANOVA with a post hoc Tukey multiple-comparison test, are indicated by different letters.