Figure 2.

The antitumor activities of the nanoparticles. (A) Mice were immunized with the nanoparticles containing bovine serum albumin (BSA) only (NP[BSA]), OVA only (NP[OVA]), both OVA and poly-IC (NP[OVA+I:C], or both OVA and CpG ODN (NP[OVA+CpG]), intravenously into tail veins of the mice. Seven days later, the mice were subcutaneously implanted with EG7.OVA tumor cells (5×10⁵/mouse). Two days later, the mice were again immunized with the same nanoparticles intravenously into tail veins of the mice. The tumor size was measured with a slide caliper and expressed as a tumor index, determined as the square root of (major axis×minor axis). (B) Mice were subcutaneously implanted with the tumor cells, and then mixtures of NP[OVA+I:C] and NP[OVA+CpG] were injected into the tumor mass on 10, 12 and 14 days after the tumor implantation.