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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7338–7348. doi: 10.4049/jimmunol.180.11.7338

Figure 1.

Figure 1

Immunohistochemistry shows high MIF expression in OvCA tissue. A, MIF expression in non-malignant and malignant ovarian tissue was analysed by immunohistochemistry using mouse anti-human MIF Mab289. Shown are representative stainings for healthy ovarian epithelium (upper left), a borderline tumor (upper right) and two OvCA (lower panels). To control for specificity, the antibody was pre-incubated with 50 μg/ml rhMIF (insert in the lower right panel). Size-bars correspond to 50 μm. B, Double stainings for MIF and tumor or macrophage markers were performed using Mab289 anti-human MIF antibody in conjunction with goat-anti-mouse Alexa Fluor 555 F(ab′)2 fragments and FITC-conjugated CD68 (left) or CD326/EpCAM antibodies. For double staining of MIF and cytokeratin 8, Mab 289 anti-human MIF (mouse IgG1κ) and CK3-3E4 anti-human cytokeratin 8 (mouse IgG2A) were visualized using isotype-specific, Alexa Fluor 488 and 555 conjugated detection antibodies. The staining shown is representative of four different tumors stained.