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. 2013 Apr;141(4):431–443. doi: 10.1085/jgp.201210942

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© 2013 Wang et al.

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Figure 5. — Isolation of Q₁ and Q₂ Q-V relations. (A) Cells were pulsed from −100 to +20 mV for 5 ms before repolarizing to a range of potentials between 0 and −120 mV. Q₁ charge was obtained by integrating off-gating currents after the 5-ms prepulse. (B) Measurement of total gating charge movement. Cells were pulsed from −100 to +60 mV for 24 ms before repolarizing to a range of potentials between +30 and −120 mV. (C) Q₁ component of Q-V_off: V_1/2–1 = −55.7 ± 2.4 mV and k₁ = 15.8 ± 0.7 mV; n = 7. (D) Q₂ charge was obtained by subtracting Q₁ from total off-gating charge after 24-ms pulses to +60 mV; V_1/2–2 = −54.2 ± 3.8 mV and k₂ = 19.4 ± 1.8 mV; n = 4.