Figure 4. Pressure elevation evokes Rho-associated kinase-mediated increase in LC₂₀ phosphorylation in rat gracilis arteries.

Representative western blot (upper) and means ±s.e.m. of LC₂₀ phosphorylation (lower) as % of total LC₂₀ at 10 (±0 Ca²⁺), 80, 120 and 140 mmHg ± Rho-associated kinase inhibition with H1152 (n= 7 with 1–2 arterial segments per sample) with monophosphorylated and unphosphorylated LC₂₀ separated by Phos-tag SDS-PAGE. #,*Significant difference (P < 0.05) from value at 10 mmHg and in control solution at each pressure, respectively.