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. 2013 Mar 11;11:18. doi: 10.1186/1478-811X-11-18

Figure 2.

Figure 2

Proliferative response of IGF-II and rhIns through IR-B. A. HeLa cells transiently transfected with pcDNA3-IR-B or EV were assayed by Western blot detecting the expression of IR and ERK 1/2 (loading control). B. MTS assay in cells transiently transfected with pcDNA3-IR-B or EV stimulated with 0.1 nM, 1.0 nM and 10.0 nM IGF-II or rhIns for 2 days. The results are shown as the mean ± s.e.m (n = 3 independent experiments). Asterisks indicate significant differences between IR and EV (p < 0.05).